Application

Research Sub CategoryImmunoglobulins & Immunology

Anti-CD63 Antibody, clone RFAC4 is an antibody against CD63 for use in FC, IP, WB, IH, IH(P).

Research CategoryInflammation & Immunology

Flow cytometric studies of CD63 expression

Immunoprecipitation

Western blotting of CD63 antigen from solubilized platelet proteins

Suitable for formalin fixed paraffin wax embedded tissue sections. HIER and proteolytic pretreatment may not be necessary. Users should confirm specificity and pretreatment for their particular application.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

The monoclonal is presented at a concentration of 100µg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Format: Purified

Specificity

This antibody reacts with a 53 kDa protein which form a part of the family of tetraspan moieties. The antigen was originally designated as a lysosomal membrane protein characterized as an activation dependent platelet surface antigen. In fact the CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma.

Storage and Stability

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.